Co-culture of rat neonatal ventricular myocytes and GFP-labeled feline c-Kit cells: Neonatal rat ventricular myocytes
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چکیده
BrdU infusion into normal cats: Osmotic pumps (ALZET) were filled with 50mg/mL BrdU dissolved in 50% DMSO/50% ddH2O filtered through a sterile 0.22μm filter. Pumps were then incubated in sterile saline at 37 °C overnight prior to insertion. The pumps were surgically inserted subcutaneously just caudal to the scapulae per the protocol approved by IACUC at Temple University. The number of pumps was varied depending on animal body weight for an average infusion of 10mg/kg/day for seven days.
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Hypoxia causes connexin 43 internalization in neonatal rat ventricular myocytes.
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